Studies of coat protein-mediated resistance to tobacco mosaic tobamovirus: correlation between assembly of mutant coat proteins and resistance
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چکیده
منابع مشابه
Induction of Resistance to Potato Virus Y (PVY) Using Hairpin Construct of Coat Protein
Potato virus Y (PVY) is one of the most damaging viruses of potato plants which infecting most cultivars and causing significant yield and economical losses. The application of the concept of pathogen derived resistance opened new horizons for the development of virus-resistant plants. This research was carried out to study RNA silencing to engineered potato plants that are resistant to potato ...
متن کاملCharacterization of mutant tobacco mosaic virus coat protein that interferes with virus cell-to-cell movement.
Expression of tobacco mosaic virus (TMV) coat protein (CP) in plants confers resistance to infection by TMV and related tobamoviruses. Certain mutants of the CP (CP(T42W)) provide much greater levels of resistance than wild-type (wt) CP. In the present work, infection induced by RNA transcripts of TMV clones that contain wt CP or mutant CP(T42W) fused to the green fluorescent protein (GFP) (TMV...
متن کاملExpression of tobacco mosaic virus coat protein and assembly of pseudovirus particles in Escherichia coli.
The bidirectional self-assembly of tobacco mosaic virus (TMV, common or U1 strain) has been studied extensively in vitro. Foreign single-stranded RNA molecules containing the TMV origin-of-assembly sequence (OAS, 75-432 nt in length) are also packaged by TMV coat protein (CP) in vitro to form helical pseudovirus particles. To study virus assembly in vivo requires an easily manipulated model sys...
متن کاملa dual coat protein construct establishes resistance to passionfruit woodiness and cucumber mosaic viruses
there is a high degree (>95%) of intraspecies similarity in the coat protein (cp) amino acid sequences within passionfruit woodiness virus (pwv) and cucumber mosaic virus (cmv), both infecting passionfruit vine in new south wales. on this basis, a dual transgene containing the translatable cdnas coding for the cps of pwv and cmv was constructed in the binary vector pbi121 and used for transform...
متن کاملThe mechanism of aggregation of the coat protein of tobacco mosaic virus. A comparative study on vulgare and mutant proteins.
The aggregation behaviour of tobacco mosaic virus (TMV) protein vulgare was compared to that of two mutants, A 14 and Ni725, with amino acid exchanges localized in the coat protein at posi tions 107 (Thr -*• Met, in Ni725) and 129 (lie -> Thr, in both mutants). This behaviour, as meas ured by sedimentation, hydrogen ion titration, light-scattering, and near-UV absorption difference and circul...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1997
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.71.10.7942-7950.1997